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KMID : 1101320130450010037
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Korean Journal of Clinical Laboratory Science
2013 Volume.45 No. 1 p.37 ~ p.42
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Cell Yield of Cerebrospinal Fluid Cell Count Using Cytocentrifuges
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Koo Bon-Kyung
Shim Hyun-Seol
Oh Jung-A
Lee Yong-Tag
Choi Dae-Yong
Lee Beom-Se
Kim Eun-Jee
Lee Seung-Tae
Kim Sun-Hee
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Abstract
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The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of 0∼5 WBCs/μL of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of 0∼5 WBCs/μL on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0∼40 cells in the 44 samples of 0 WBC/μL, and 3∼95 cells in the 31 samples of 1 WBC/μL. It was observed that the nucleated cell number for cells recovered on slide was 13∼100 cells in the 44 samples of 2 WBCs/μL, and more than 100 cells in the 29 samples of 3∼5 WBCs/μL, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of 0∼5 WBCs/μL. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as 1 WBC/μL in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at 0 WBC/μL by using the cell yield in a comparison between the value of 0∼5 WBCs/μL and nucleated cell number for cells recovered on slide.
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KEYWORD
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Cell yield, Cerebrospinal fluid, Cell count, Cytocentrifugation
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